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by flow cytometry. Data are indicated as mean ± SD (n = 3). 3.3 The effect of NaCl hypertonicity treatment on chitosan transfection Next, we tested whether hypertonicity induced by the compound without the ability to penetrate cell membranes could improve chitosan/DNA polyplexes transfection efficiency. We examined the Flow Cytometry/Cell Sorting, and Biostatistics shared resources sup-ported by USPHS Grant 1P30-CA-51008. 2 To whom requests for reprints should be addressed, at Department of Cell Biology and Lombardi Cancer Center. Georgetown University Medical School, Research Building W3l6, 3970 Reservoir Road, NW, Washington. DC 20007. Next, the cellular ROS level was measured by flow cytometry as described previously . As shown in Figure 1(c) , the populations in higher fluorescence intensities significantly increased by ~10% after the exposure of DpdtpA to the cells for 24 h, but the addition of NAC, a ROS scavenger, significantly decreased ROS production (~23%), hinting ... • Suitable for fluorescence microscopy and flow cytometry applications HeLa cells were stained with mitochondrial dye MitoLite™ Green (Cat#22666, Green). After fixation in 4% formaldehyde, F-actin was co-stained with Phalloidin-iFluor™ 633 (Cat#23125, Red) and co-stained with nuclei dye Nuclear Blue™ DCS1 (Cat#17548, Blue). The aim of this study was to use the selective accumulation of LysoTracker in lamellar bodies as a method for subsetting viable AT2 cells by flow cytometry, and tracking their differentiation in live-cell culture by microscopy.
Measurement of autophagy by flow cytometry (Gary Warners): In recent years, flow cytometry has been used to detect the presence of autophagy mainly by the fluorescent antibody labeling of the autophagy marker LC3-II. Here we describe the use of the indirect antibody labeling of LC3-II as well as LysoTracker dyes to investigate the autophagic ...
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Tuberculosis is an important health problem worldwide. The only available vaccine is M. bovis/BCG, an attenuated mycobacterium that activates the innate and the acquired immune system after being phagocytosed by macrophages and dendritic cells. Vaccination fails to prevent adult pulmonary tuberculosis although it may have a protective effect in childhood infection. Understanding how BCG ... 2010 chrysler 300c srt8.
Measurement of autophagy by flow cytometry (Gary Warners): In recent years, flow cytometry has been used to detect the presence of autophagy mainly by the fluorescent antibody labeling of the autophagy marker LC3-II. Here we describe the use of the indirect antibody labeling of LC3-II as well as LysoTracker dyes to investigate the autophagic ... As in LysoTracker® dye-stained cells, the lysosomal fluorescence in LysoSensor™ dye-stained cells may constitute only a small por-tion of total cellular fluo resc ence, making it difficult to quantitate the number of lysos omes or their pH by flow cytometry or fluorometry. Table 2. Summary of our LysoTracker® and LysoSensor™ probes.